| 碱蓬cDNA表达文库的构建 |
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| 引用本文： | 马秀灵 王丽萍 等. 碱蓬cDNA表达文库的构建[J]. 西北植物学报, 2002, 22(6): 1289-1294 |
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| 作者姓名： | 马秀灵 王丽萍 等 |
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| 作者单位： | 山东师范大学逆境植物重点实验室,济南,250014 |
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| 基金项目： | 国家重点基础研究专项资金资助项目 ( G19990 1170 0 ) |
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| 摘 要： | 以盐生植物盐地碱蓬（Suaeda salsa)地上部分为材料，提取植物总RNA，纯化出mRNA，合成cDNA第一链，得到双链cDNA后，连接入植物表达载体，构建cDNA表达文库。该文库重组子约10^6。将该文库转化农杆菌GV3101，可直接用以转化拟南芥。
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| 关 键 词： | cDNA表达文库 表达载体 盐地碱蓬 耐盐性 抗逆性 |
Construction of a cDNAexpression library of Suaeda salsa |
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| Abstract. Construction of a cDNAexpression library of Suaeda salsa[J]. Acta Botanica Boreali-Occidentalia Sinica, 2002, 22(6): 1289-1294 |
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| Authors: | Abstract |
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| Abstract: | In order to find new genes related to salt stress,we constructed a Suaeda salsa aerial part tissue cDNA expression library.Halophyte S.salsa was used as plant material,the first strands of cDNAs were synthesized from mRNA.Ligated the cDNAs into the expression vector,then transformed Escherichia.coli .The library titer is about 10+6.Plasmids were extracted from amplified library and were transferred into Agrobacterium ,which subsequently was used to transform Arabidopsis thaliana . |
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| Keywords: | cDNA expression library expression vector Suaeda salsa |
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