Steatosis-induced proteomic changes in liver mitochondria evidenced by two-dimensional differential in-gel electrophoresis |
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Authors: | Douette Pierre Navet Rachel Gerkens Pascal de Pauw Edwin Leprince Pierre Sluse-Goffart Claudine Sluse Francis E |
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Affiliation: | Laboratory of Bioenergetics, Bat. B6c, Allée de la Chimie 3, 4000 Liège, Belgium. |
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Abstract: | Steatosis encompasses the accumulation of droplets of fats into hepatocytes. In this work, we performed a comparative analysis of mitochondrial protein patterns found in wild-type and steatosis-affected liver using the novel technique two-dimensional differential in-gel electrophoresis (2D-DIGE). A total of 56 proteins exhibiting significant difference in their abundances were unambiguously identified. Interestingly, major proteins that regulate generation and consumption of the acetyl-CoA pool were dramatically changed during steatosis. Many proteins involved in the response to oxidative stress were also affected. |
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