Subculture on potato dextrose agar as a complement to the broth microdilution assay for Malassezia pachydermatis |
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Authors: | Prado Marilena R Brito Erika H S Brilhante Raimunda S N Cordeiro Rossana A Leite João J G Sidrim José J C Rocha Marcos F G |
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Institution: | Faculty of Veterinary Medicine, Program of Post-Graduation in Veterinary Science, State University of Ceará, Fortaleza, Ceará, Brazil. |
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Abstract: | The main aim of this study was to verify the efficacy of subculture on potato dextrose agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of <0.03, <0.03, 2.0, 4.0 and 4.0 mug/ml for itraconazole, ketoconazole, voriconazole, fluconazole and amphotericin B, respectively. Thus, the subculture on PDA improved the analysis of the in vitro antifungal susceptibility of M. pachydermatis. |
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