Synergistic effect of amino acids on production of lymphocyte proliferation-suppressing substances by rat intestinal epithelial cells |
| |
Authors: | Noritsugu Yabe Yasuharu Itagaki Morimasa Tanimoto Hisao Matsui |
| |
Affiliation: | (1) Department of Hygiene, Dokkyo University School of Medicine, Mibu, Tochigi, 321-0293, Japan;(2) The Nutritional Science Laboratory, Snow Brand Milk Products Co., Kawagoe, Saitama, 350-1165, Japan |
| |
Abstract: | Conditioned culture medium of rat small intestinal epithelial cells suppressed proliferation of spleen lymphocytes stimulated with concanavalin A (approx. 10% of its control [3H]thymidine incorporation) whereas conditioned phosphate-buffered saline of the epithelial cells did not. On the other hand, conditioned saline of the epithelial cells exposed to a mixture of total 22 amino acids at their concentrations in the culture medium suppressed the proliferation (approx. 45% of its control [3H]thymidine incorporation). Neither conditioned saline of the epithelial cells exposed to other medium components nor lysates of freshly harvested epithelial cells suppressed the proliferation. Thus, amino acids synergistically stimulated intestinal epithelial cells to produce substances with the ability to suppress lymphocyte proliferation. |
| |
Keywords: | amino acid intestinal epithelial cells lymphocyte proliferation suppression |
本文献已被 SpringerLink 等数据库收录! |
|