PERMANENT GENETIC RESOURCES: Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum |
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| Authors: | Giraud T Yockteng R Marthey S Chiapello H Jonot O Lopez-Villavicencio M DE Vienne D M Hood M E Refregier G Gendrault-Jacquemard A Wincker P Dossat C |
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| Affiliation: | ESE, Batiment 360, Université Paris-Sud, 91405 Orsay, France, MIG-INRA Batiment 233, Domaine de Vilvert, 78350 Jouy en Josas Cedex, France, Department of Biology, Amherst College, Amherst, MA 01002, USA, Genoscope-CNRG CP 5706, 91057 Evry Cedex, France. |
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| Abstract: | We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum, causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis-dioicae, M. violaceum sensus stricto, M. lagerheimii and M. dianthorum, collected, respectively, from the plants Silene latifolia, S. nutans, S. vulgaris and Dianthus carthusianorum. Intrapopulation polymorphism was investigated using 24 isolates, and cross-amplification was explored using 23 isolates belonging to at least 10 different Microbotryum species. This study provides numerous microsatellite markers for population genetics and mapping studies. |
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| Keywords: | basidiomycete phytopathogen repeats transposable elements Ustilago violacea |
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