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A semiautomated method for determination of NADH-methemoglobin reductase activity
Authors:J C Standefer  E J Matusik  J I Davis  J E Matusik
Affiliation:1. Food Technology Department, School of Biotechnology, International University, Ho Chi Minh City, Viet Nam;2. Vietnam National University, Ho Chi Minh City, Viet Nam;3. Food Group, Department of Wine, Food and Molecular Biosciences, Agriculture and Life Sciences, Lincoln University, Canterbury, New Zealand;1. Universidad de Santiago de Chile, Ave. Lib. Bdo ÓHiggins 3363, Santiago de Chile, Chile;2. Instituto de la Grasa (CSIC), Campus Universitario Pablo de Olavide - Edificio 46, Ctra. de Utrera, km. 1, 41013 Sevilla, Spain;3. Ministerio de Ciencia y Tecnología, Calle 2 No 124 e/ 1ra y 3ra Miramar, La Habana, Cuba;4. Leibniz Institute for Agricultural Engineering and Bioeconomy e.V. (ATB), Max-Eyth-Alle 100, 14469 Potsdam, Germany;1. Department of Food and Nutritional Sciences, Sri Sathya Sai Institute of Higher Learning (Deemed to be University), Anantapur, 515001, Andhra Pradesh, India;2. Department of Chemistry, Sri Sathya Sai Institute of Higher Learning (Deemed to be University), Prasanthi Nilayam, 515134, Andhra Pradesh, India;1. Hospital Regional de Alta Especialidad de la Península de Yucatán, Yucatán, México;2. Departamento de Metodología de la Investigación, Instituto Nacional de Pediatría, México, México;3. Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Ciudad de, México
Abstract:A semiautomated method for measuring NADH-methemoglobin reductase is presented. The method is patterned after the manual method of Hegesh et al. (2). Hemoglobin substrate concentration is 0.50 gm/ 100 ml and a 1:30 dilution of packed red blood cells serves as the enzyme source. Fifty samples per hour may be measured using as little as 5 μl of packed cells. The precision and accuracy of this method compares favorably with the manual methods of Hegesh et al. (2) and Scott (1).
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