In vitro cold storage of cork oak shoot cultures |
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| Authors: | Romano Anabela Martins-Loução Maria Amélia |
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| Institution: | (1) Institute for Plant Virology, Microbiology and Biosafety, Federal Biological Research Centre for Agriculture and Forestry, Messeweg 11/12, D-3810 Braunschweig, Germany;(2) Department of Biochemistry and Molecular Biology, Federal University of Ceará, P.O. Box 1065, 60001-970 Fortaleza, CE, Brazil |
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| Abstract: | Friable callus cultures were initiated from cotyledons and hypocotyls of Opuntia ficus-indica. Explants from cotyledons produced significantly more callus than those from hypocotyls. Optimum callus growth was observed
on Murashige & Skoog medium supplemented with 0.9 μM 6-furfurylaminopurine, 2.3 μM 2,4-dichlorophenoxyacetic acid, 1.0 μM
4-amino 3,5,6-trichloropicolinic acid, 400 mg l-1 casein hydrolysate and 3% sucrose. The same medium without agar was used for establishing cell suspensions.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | Cactaceae forage crops in vitro propagation |
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