Variability in messenger RNA levels in human umbilical vein endothelial cells of different lineage and time in culture |
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Authors: | David Dichek Thomas Quertermous |
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Affiliation: | (1) Cardiac Unit, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Jackson 12, 02114 Boston, Massachusetts |
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Abstract: | Summary Levels of seven messenger RNA species were compared in human umbilical vein endothelial cells of different lineage and time in culture. Specifically, cells obtained from the American Type Culture Collection (ATCC) and subcultured were compared to early passage cells from cultures produced in our laboratory. Messenger RNA for tissue plasminogen activator, plaminogen activator inhibitor 1, urokinase, and thrombomodulin were expressed at higher levels in the ATCC cells. Thrombospondin, von Willebrand's Factor, and protein S messenger RNA were expressed at higher levels in the cells that we isolated. In addition, in the ATCC cells a shift in the proportion of plasminogen activator inhibitor messenger RNA from the 3.4 to the 2.4 kilobase species was found. We conclude that specific messenger RNA levels can vary considerably between cultured human umbilical vein endothelial cells. The large variation in mRNA levels which we describe has important implications for experiments involving gene expression in cultured endothelium. |
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Keywords: | vascular endothelium messenger RNA gene expression regulation |
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