UV-B诱导的酵母凋亡现象及调节机制的作用

研究UV-B诱导的酵母凋亡现象及调节机制的作用。通过高密度细胞培养, UV-B能够抑制酵母细胞生长和诱导细胞凋亡。然而, 将UV-B已照射96 h活酵母细胞重新进行UV-B照射时发现, 培养12 d照射过细胞的存活率仍有10% (P<0.05), 而未照射细胞已经基本死亡。同时, 经0.01 mol/L和0.1 mol/L H2O2处理, UV-B照射24 h活细胞的存活率分别是对照的3.0倍和5.2倍; 而经30 min和60 min 55°C热处理, UV-B照射24 h活细胞的存活率分别是对照的3.5倍和9.0倍。

UV-B Irradiation Regulates Apoptosis in Yeast

Mechanisms of UV-B-induced apoptotic regulation in yeast Saccharomyces cerevisiae were studied. The results showed that UV-B irradiation indeed inhibited the growth of yeast cells as well as induced extensive apoptosis during 96 h experiment period. However, survival of 96 h irradiated cells remained 10% while most control cells finally dead after re-growth under UV-B irradiation for 12 d. And by exposed to 0.01 mol/L or 0.1 mol/L H2O2 for 30 min, survival rate of 24 h irradiated cells were 3.0-fold or 3.2-fold than control, respectively. By to heat shock for 30 min or 60 min, survival rate of 24 h irradiated cells were 3.5-fold or 9.0-fold than control, respectively.