Structural analyses of lipid A from lipopolysaccharides of nodulating and non-nodulating Rhizobium trifolii |
| |
Authors: | Ryszard Russa Otto Lüderitz Ernst Th. Rietschel |
| |
Affiliation: | (1) Max-Planck-Institut für Immunbiologie, D-7800 Freiburg, Federal Republic of Germany;(2) Forschungsinstitut Borstel, D-2061 Borstel, Federal Republic of Germany;(3) Present address: Institute of Microbiology, M. Curie-Sklodowska University, PL-20-033 Lublin, Poland |
| |
Abstract: | Structural studies have been performed on lipid A preparations derived from lipopolysaccharides of nodulating (nod+) Rhizobium trifolii wild type strains and non-nodulating (nod-) mutants deprived of the medium size Nod plasmid.All preparations contain a lipid A backbone composed of a 1,6-linked d-glucosamine disaccharide (GlcN II-1,6-GlcN I) which is bis-phosphorylated at positions 1 and 4. The phosphate group at position 4 (GlcN II) is nonstoichiometrically substituted probably by a neutral glycosyl residue. Another substituent (probably also a neutral sugar) substitutes partly position 4 (GlcN I) of the disaccharide. The hydroxyl groups at positions 3 and 3 are likely to be esterified by fatty acyl residue. In lipid A of nod+ strains, 3-hydroxyhexa- and octadecanoic acid are linked to the amino group of GlcN I, and 3-hydroxyhexa- and tetradecanoic acid to the amino group of GlcN II. In lipid A of nod- strains, mainly 3-hydroxyhexadecanoic acid is linked to the amino group of GlcN I, and 3-hydroxytetra- and hexadecanoic acid to that of GlcN II.The results show that rhizobial lipid A expresses many similarities to the lipid A of Enterobacteria. The structure shows, however, also peculiarities, especially regarding substituents of the lipid A backbone.This paper is dedicated to Professor Dr. Gerhart Drews on the occasion of his 60th birthday |
| |
Keywords: | Rhizobium trifolii Lipopolysaccharide Lipid A-structure /content/p121273332351w40/xxlarge8220.gif" alt=" ldquo" align=" MIDDLE" BORDER=" 0" >Nod /content/p121273332351w40/xxlarge8221.gif" alt=" rdquo" align=" MIDDLE" BORDER=" 0" > plasmids 3-Hydroxy fatty acids |
本文献已被 SpringerLink 等数据库收录! |
|