Essential Oil of Zingiber cassumunar Roxb. and Zingiber officinale Rosc.: A Comparative Study on Chemical Constituents,Antibacterial Activity,Biofilm Formation,and Inhibition of Pseudomonas aeruginosa Quorum Sensing System |
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Authors: | Abdi Wira Septama Monika Arvia Chiara Gabriel Turnip Aprilia Nur Tasfiyati Rizna Triana Dewi Erlia Anggrainy Sianipar Amit Jaisi |
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Institution: | 1. Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Banten 15314 Indonesia;2. Department of Pharmacy, School of Medicine and Health Science, Atma Jaya Catholic University of Indonesia, 14440 Jakarta, Indonesia;3. Research Center for Chemistry, National Research and Innovation Agency (BRIN), Kawasan PUSPIPTEK Serpong, Tangerang Selatan, Banten 15314 Indonesia;4. School of Pharmacy, Walailak University, Thasala, Nakhon Si Thammarat, 80160 Thailand |
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Abstract: | Pseudomonas aeruginosa can regulate its pathogenicity via quorum sensing (QS) system. Zingiber cassumunar and Z. officinale have been used for the treatment of infectious diseases. The study aimed to evaluate and compare the chemical constituents, antibacterial, and QS inhibitor of Z. cassumunar essential oils (ZCEO) and Z. officinale essential oils (ZOEO). The chemical constituent was analysed using GC/MS. Broth microdilution and spectrophotometry analysis were used to evaluate their antibacterial and QS inhibitor activities. The main constituent of ZOEO with percent composition above 6 % (α-curcumene, α-zingiberene, β-sesquiphellandrene, and β-bisabolene, α-citral, and α-farnesene) were exist in a very minimal percentage less than 0.7 % in Z. cassumunar. All major components of ZCEO with percentages higher than 5 % (terpinen-4-ol, sabinene, γ-terpinene) were present in low proportion (<1.18 %) in Z. officinale. ZCEO demonstrated moderate antibacterial activity against P. aeruginosa. The combination of ZCEO and tetracycline showed a synergistic effect (FICI of 0.5). ZCEO exhibited strong activity in inhibiting biofilm formation. ZCEO at MIC (62.5 μg/mL) was able to reduce pyoverdine, pyocyanin, and proteolytic activity. This is the first report on the activity of ZCEO in the inhibition of P. aeruginosa QS system and it may be used to control the pathogenicity of P. aeruginosa. |
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Keywords: | antibacterial essential oil virulence factor Z cassumunar Z officinale |
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