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UHPLC-Q-Orbitrap HR-MS-Based Metabolomics for Profiling the Sida rhombifolia Metabolites with Different Plant Organs and Cultivation Ages |
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| Authors: | Dea Silviani Safirah Zam Astari Dewi Anggraini Septianingsih Alfi Hudatul Karomah Auliya Ilmiawati Utami Dyah Syafitri Wulan Tri Wahyuni Nanik Siti Aminah Muhamad Insanu Abdul Rohman Mohamad Rafi |
| Institution: | 1. Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680 Indonesia;2. Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680 Indonesia
Advanced Research Laboratory – Institute of Research and Community Service, IPB University, Jalan Palem Raya, Kampus IPB Dramaga, Bogor, 16680 Indonesia;3. Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680 Indonesia Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3, Bogor, 16128 Indonesia;4. Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3, Bogor, 16128 Indonesia Department of Statistics, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Meranti kampus IPB Dramaga, Bogor, 16680 Indonesia;5. Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Jl. Mulyorejo Kampus C Unair, Surabaya, 60286 Indonesia;6. School of Pharmacy, Bandung Institute of Technology, Jalan Ganesha No. 10, Bandung, 40132 Indonesia;7. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, 55281 Indonesia |
| Abstract: | Plant organs and cultivation ages can result in different compositions and concentration levels of plant metabolites. The metabolite profile of plants can be determined using liquid chromatography. This study determined the metabolite profiles of leaves, stems, and roots of Sida rhombifolia at different cultivation ages at 3, 4, and 5 months post-planting (MPP) using liquid chromatography-mass spectrometry/mass spectrometry (LC/MS/MS). The results identified that 41 metabolites in S. rhombifolia extract for all plant organs and cultivation ages. We successfully identified approximately 36 (leaves), 22 (stems), and 18 (roots) compounds in all extract. Using principal component analysis (PCA) with peak area as the variable, we clustered all sample extracts based on plant organs and cultivation ages. As a result of PCA, S. rhombifolia extracts were grouped according to plant organs and cultivation ages. In conclusion, a clear difference in the composition and concentration levels of metabolites was observed in the leaves, stems, and roots of S. rhombifolia harvested at 3-, 4-, and 5-MPP. |
| Keywords: | cultivation ages plant organs Sida rhombifolia UHPLC?Q-Orbitrap untargeted metabolomics |