Effect of incubation temperature after devitrification on quality parameters in human sperm cells |
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| Affiliation: | 1. Center of Excellence in Translational Medicine - Scientific and Technological Bioresource Nucleus (CEMT - BIOREN), Chile;2. Center of Reproductive Biotechnology - Scientific and Technological Bioresource Nucleus (CEBIOR – BIOREN), Chile;3. Department of Internal Medicine, Chile;4. Department of Preclinical Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile;5. School of Medical Technology, Faculty of Science, Universidad Mayor, Temuco, Chile;6. Research Group for Reproductive Medicine, Department of Obstetrics and Gynaecology, Cologne University, Cologne, Germany;1. Texas Tech University, 2500 Broadway, Lubbock, TX 79409, United States;2. Texas Tech University Health Sciences Center, 3601 4th St, Lubbock, TX 79430, United States;1. State Key Laboratory for Advanced Metals and Materials, University of Science and Technology Beijing, Beijing 100083, PR China;2. Laboratory of Applied Physics and Mechanics of Advanced Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China;3. Institute of Applied Mechanics and Biomedical Engineering, Taiyuan University of Technology, Taiyuan 030024, PR China;1. Universitair Ziekenhuis Brussel (UZ Brussel), Centrum voor Reproductieve Geneeskunde, Laarbeeklaan 101, 1090 Brussels, Belgium;2. Central Queensland University, Bruce Highway, North Rockhampton QLD 4702, Australia |
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| Abstract: | Sperm cryopreservation is common in assisted reproduction laboratories, providing a therapeutic option for several clinical conditions. This process has been optimized; however, the effect of post-thaw incubation temperature has been poorly studied. This work analyzed the effect of incubation temperature after devitrification on human sperm quality. Spermatozoa from normozoospermic donors were cryopreserved by vitrification. After devitrification, the spermatozoa were separated into two aliquots: (i) incubated at room temperature (RT, 22-25 °C) and (ii) incubated at 37 °C. Reactive oxygen species (ROS), viability, mitochondrial membrane potential (ΔΨM), phosphatidylserine externalization and motility were analyzed immediately after devitrification (control) and after 2, 4 and 6 h. Spermatozoa incubated at RT showed a conserved viability and ΔΨM compared to the control, while the incubation at 37 °C promoted a decrease in these parameters. The ROS levels were increased at both incubation conditions. The progressive motility was decreased in all experimental groups and the decrease was more pronounced under incubation at RT. No increase in phosphatidylserine externalization was observed. In conclusion, prior to use in assisted reproduction procedures, devitrified spermatozoa at RT conserve a better viability and ΔΨM than at 37 °C. |
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| Keywords: | Sperm cryopreservation Vitrification Human sperm Reactive oxygen species |
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