| Institution: | 1. Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming, Yunnan, 650224 China;2. Key Laboratory of National Forestry and Grassland Administration on Highly-Efficient Utilization of Forestry Biomass Resources in Southwest China, Southwest Forestry University, Kunming, Yunnan, 650224 China
These authors contributed equally to this work and should be considered co-first authors.;3. Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Southwest Forestry University, Kunming, Yunnan, 650224 China;4. Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing, Jiangsu, 210042 China;5. Yunnan Agricultural University, Kunming, Yunnan, 650201 China |
| Abstract: | Subcritical water extraction was used to extract bioactive phenolic compounds from Vaccinium dunalianum Wight leaves. The optimal extraction conditions were determined as an extraction temperature of 150 °C, an extraction time of 40 min, and a liquid-solid ratio of 35 : 1 mL/g. The total phenolic content reached 21.35 mg gallic acid /g, which was 16 % higher than that by hot water extraction. The subcritical water extraction extract exhibited strong scavenging activity of DPPH free radical and ABTS+ free radical, as well as significant tyrosinase inhibitory activity. The study suggests that subcritical water extraction can alter the composition of the extracts, leading to the production of various phenolic compounds, effective antioxidants, and tyrosinase inhibitors from Vaccinium dulciana Wight leaves. These findings confirm the potential of Vaccinium dunalianum Wight as a natural antioxidant molecule source for the medicine and food industries, and for the therapy of skin pigmentation disorders. |
