Phytochemical Compounds of Euphorbia bivonae Extract and Their Cytotoxicity Effects on the Lethality of Brine Shrimp Artemia salina and Embryonic Kidney (HEK293) Cells |
| |
| Authors: | Hanen Enneb Khaled Athmouni Rahma Thabet Habib Ayadi |
| |
| Institution: | 1. Laboratory of Dryland and Oasis Cropping, Institute of Arid Zone of Médenine, University of Gabes, ElFjè, Medenine 4119 Tunisia;2. Laboratory of Biodiversity and Aquatic Ecosystems UR/11ES72, Ecology and Planktonology, Department of Life Sciences, University of Sfax, Soukra Road Km 3.5, BP1171, 3000 Sfax, Tunisia |
| |
| Abstract: | In this research article, we investigated the effect of Euphorbia bivonae extract compounds on the lethality of brine shrimp Artemia salina and on embryonic cell lines (HEK293) proliferation. Our GC/MS analysis revealed that the E. bivonae ethanolic extract contained essentially sitosterol, euphol, and lupeol. The 24-h LC50 was determined using the probit analysis method (LC50=357.11 mg l−1). Depending on this cytotoxicity test result, E. bivona extract induced a significant increase in Superoxide Dismutase (SOD), Catalase (CAT), Glutathione-Peroxidase (GPx) activities, and lipid peroxidation (LPO) in A. salina larvae. In addition, the cytotoxicity effect of this extract had proved against the HEK293 cell lines in vitro. We suggest that the three compounds of E. bivonae extract (sitosterol, euphol, and lupeol) are the most responsible for this cytotoxicity. The possible application of this extract as an alternative natural antiproliferative is considered. |
| |
| Keywords: | A salina larvae cytotoxicity E bivonae extract GC/MS analysis HEK293 cell lines oxidative biomarkers |
|
|
