Effect of lecithin nanoliposome or soybean lecithin supplemented by pomegranate extract on post-thaw flow cytometric,microscopic and oxidative parameters in ram semen |
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| Institution: | 1. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;2. Department of Food Science and Technology, College of Agriculture, University of Tabriz, Tabriz, Iran;1. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;2. Department of Veterinary Pre-Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran;3. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;4. Department of Research, Breeding and Production of Laboratory Animals, Rzai Vaccine and Serum Research Institute, Karaj, Iran;1. Animal Science Department, College of Agriculture, Isfahan University of Technology, P.O.Box: 84156/83111, Isfahan, Iran;2. Department of Reproductive Biotechnology, Reproductive Biotechnology Research Center, Avicenna Research Institute ACECR, Shahid Beheshti University, Tehran, Iran;3. Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;1. INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco;2. Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan 1, BP 577, 26000 Settat, Morocco;3. INRA, UMR 85 Physiologie de la Reproduction et des Comportements, F-37380 Nouzilly, France;4. Departments of Chemistry, Faculty of Science, Mu?la S?tk?Koçman University, 48121 Mu?la, Turkey;1. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;2. Department of Clinical and Experimental Medicine (IKE), Division of Clinical Sciences, Obstetrics and Gynecology, Linköping University, Linköping, Sweden;1. Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran;2. Biomedical Center, Medical Faculty in Pilsen, Charles University in Prague, Alej, Svobody 1655/76, 323 00 Pilsen, Czechia |
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| Abstract: | This investigation was carried out to study the effect of soybean lecithin 1.5% (wt/vol) (0, 2.5, 5 and 7.5 mg l?1 pomegranate extract (PE)) or PE-loaded lecithin nanoliposome (0, 2.5, 5 and 7.5 mg l?1) to Tris-based extender. Sperm motility (CASA), viability, membrane integrity (HOS test), abnormalities, mitochondrial activity, apoptosis status, lipid peroxidation, total antioxidant capacity (TAC)) and antioxidant activities (GPX, SOD) were investigated following freeze-thawing. No significant differences were detected in motility parameters, viability, membrane integrity, and mitochondria activity after thawing sperm between soybean lecithin and lecithin nanoliposomes. It was shown that PE5 significantly improved sperm total and progressive motility, membrane integrity, viability, mitochondria activity, TAC and reduced lipid peroxidation (malondialdehyde concentration). Moreover, the percentage of apoptotic sperm in PE5 extenders was significantly the lowest among other treatments. Sperm abnormalities, SOD and GPX were not affected by the antioxidant supplements. For apoptotic status, no differences were observed between soybean lecithin and lecithin nanoliposome. We showed that lecithin nanoliposome extender can be a beneficial alternative extender to protect ram sperm during cryopreservation without any adverse effects. It was also observed that regarding pomegranate concentration, PE5 can improve the quality of ram semen after thawing. |
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| Keywords: | Lecithin nanoliposome Soybean lecithin Pomegranate extract Ram sperm Cryopreservation |
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