|
|||||
|
|
Inhibitory Effects of Corydalis saxicola Bunting Total Alkaloids on Macrophage Pyroptosis |
|
| Authors: | Dr Cong Feng Dr Xiangzhi Yong Dr Qiaozhi Jiang Prof Zhiheng Su Dr Zhenmin Liu Dr Tiantian Wu Prof Renchuan Tao |
| Institution: | 1. Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, P.?R. China
Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, P.?R. China Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, P.?R. China Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, P.?R. China Cong Feng and Xiangzhi Yong contributed equally to this work.;2. Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, P.?R. China Guangxi Health Commission Key Laboratory of Prevention and Treatment for Oral Infectious Diseases, Nanning, P.?R. China Guangxi Key Laboratory of Oral and Maxillofacial Rehabilitation and Reconstruction, Guangxi Universities Colleges Key Laboratory of Oral and Maxillofacial Surgery Disease Treatment, Guangxi Clinical Research Center for Craniofacial Deformity, Nanning, P.?R. China Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, P.?R. China;3. Pharmaceutical College, Guangxi Medical University;4. Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, P.?R. China Guangxi Key Laboratory of AIDS Prevention and Treatment, Guangxi Medical University, Nanning, P.?R. China;5. Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, P.?R. China |
| Abstract: | This study investigated the effect of Corydalis saxicola Bunting total alkaloids (CSBTA) on pyroptosis in macrophages (M?). In the M? pyroptosis model, an inverted fluorescence microscope was used to assess cell pyroptosis, while a scanning electron microscope was used to observe morphological changes in M?. NLR family pyrin domain-containing 3 (NLRP3), caspase-1, and gasdermin D (GSDMD) expression levels were detected by polymerase chain reaction and western blotting, whereas interleukin-1 (IL-1) and interleukin-18 (IL-18) expression levels were measured by an enzyme-linked immunosorbent assay. After pretreatment with CSBTA or the caspase-1 inhibitor, acetyl-tyrosyl-valyl-alanyl-aspartyl-chloromethylketone (Ac-YVAD-cmk), it was discovered that NLRP3, caspase-1, and GSDMD expressions were significantly reduced at both the mRNA and protein levels, as were IL-1 and IL-18 levels. The inhibitory effects of CSBTA and Ac-YVAD-cmk did not differ significantly. These findings indicate that CSBTA blocks Porphyromonas gingivalis-lipopolysaccharide-induced M? pyroptosis. |
| Keywords: | Corydalis saxicola Bunting total alkaloid macrophages pyroptosis |