Phytochemical and Cytogenetic Study of Two Centaurea Species from Croatia: the Particular Case of Diploid and Tetraploid C. salonitana |
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| Authors: | Ivana Carev Mirko Ruščić Mateo Glumac Olivera Politeo Sonja Siljak-Yakovlev |
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| Institution: | 1. Department of Biochemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia;2. Department of Biology, Faculty of Natural Sciences, University of Split, Ruđera Bokovića 33, 21000 Split, Croatia;3. Department of Cancer Research, Medical School, University of Split, Šoltanska ulica 2, 21000 Split, Croatia;4. Ecologie Systématique Evolution, Univ. Paris-Sud, CNRS, AgroParisTech, Université Paris-Saclay, 91190 Gif-sur-Yvette, France |
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| Abstract: | Natural wild populations of C. rupestris and C. salonitana were studied to determine possible relationships between the volatile oil (VO) composition and ploidy level. The chemical composition of the volatile oil was investigated using the GC/MS technique. The predominant components of the VO of diploid and tetraploid C. salonitana were hexadecanoic acid and α-linoleic acids, while in C. rupestris they were germacrene D and β-caryophyllene in one population and heptacosane and germacrene D, in another. The nuclear DNA amounts (2 C DNA), determined by flow cytometry, were 3.54 pg for C. rupestris, 3.39 pg for the diploid and 6.79 pg for the tetraploid population of C. salonitana. Evidence that the degree of ploidy solely influences the chemical composition of the essential oil of C. salonitana was not found. The results presented are the first data to be reported on the DNA content of the studied Centaurea populations from Croatia, as well as on the chemical composition of C. salonitana volatile oil. |
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| Keywords: | Centaurea salonitana Centaurea rupestris essential oil chemical composition GC/MS genome size |
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