Vitamin K2 improves developmental competency and cryo-tolerance of in vitro derived ovine blastocyst |
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| Institution: | 1. Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran;2. Department of Biology, Faculty of Science, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran;1. Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA;2. Department of Engineering, East Carolina University, Greenville, NC, USA;3. Institute for Engineering in Medicine, University of Minnesota, Minneapolis, MN, USA;4. Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA;1. Clinica Santa Elena, Malaga, Spain;2. Inter Science GmbH, Biophysics, Luzern, Switzerland;3. Instituto Andaluz Cirugía Experimental IACE, Malaga, Spain;4. Hippocrates DOO, Divaca, Slovenia;5. Pathology Research Laboratory, Inc, 521 Rocca Avenue, South San Francisco, CA 94080, USA;6. Department of Mechanical Engineering, University of California, Berkley, CA 94720, USA;1. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia;2. Aquatic Sciences, South Australian Research and Development Institute, 2 Hamra Avenue, West Beach, SA 5024, Australia;3. Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, Dinajpur 5200, Bangladesh;1. National Research Institute of Far Seas Fisheries, Japan Fisheries Research and Education Agency, 2-12-4 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-8648, Japan;2. Tohoku National Fisheries Research Institute, Japan Fisheries Research and Education Agency, 25-259, Shimo-mekurakubo, Same-machi, Hachinohe, Aomori 031-0841, Japan;3. National Fisheries University, Japan Fisheries Research and Education Agency, 2-7-1, Nagata-honmachi, Shimonoseki, Yamaguchi 759-6595, Japan;4. Marine Fisheries Research and Development Center, Japan Fisheries Research and Education Agency, 2-3-3 Minatomirai, Nishi-ku, Yokohama, Kanagawa 220-6115, Japan |
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| Abstract: | Vitamin K2 (VK2), acts as an electron carrier in mitochondria and thereby effects reactive oxygen species (ROS) and ATP production. This study evaluates role of VK2 on in vitro developmental competency and cryo-survival of pre-implantation ovine embryos. Initially the optimal and beneficial concentration of VK2 on compaction and blastocyst formation rates was defined (0.1 μM). Subsequently, it was shown that 0.1 μM VK2, at blastocyst stage, reduces H2O2 production, increase the expression of mitochondrial related gene and improved embryos quality. We further assessed presence VK2 supplementation before and/or after vitrification of in vitro derived blastocysts. Our results reveal that presence of VK2 before and after vitrification improves rates of blastocysts re-expansion (88.19± 3.37% vs 73.68± 1.86%, P < 0.05) and hatching (49.55± 4.37% vs 32.7± 3.32%) compared to control group. These observation were consistent with reduction in H2O2 production and improved in expression of mitochondrial related genes. However, VK2 before or after vitrification, not only had no positive effect on these two parameters, but also significantly reduced these parameters. Therefore, in concordance with pervious report in bovine, we show that VK2 supplementation post genomic activation (Day 3–7) improved developmental competency of ovine in vitro derived embryos. We also showed that presence of VK2 after vitrification improves the cryo-survival of ovine embryos. |
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| Keywords: | Vitamin K2 Vitrification Blastocyst Reactive oxygen species Mitochondrial |
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