Effect of storage conditions on the LDL effectiveness in ovine sperm cryopreservation |
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| Institution: | 1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Institute of Aquaculture and Protection of Waters, Husova t?. 458/102, 370 05 ?eské Budějovice, Czech Republic;2. Department of Ichthyobiology and Fisheries, University of Agriculture, ul. Prof. T. Spiczakowa 6, Mydlniki, 30-199 Krakow, Poland;3. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Záti?í 728/II, 389 25 Vodňany, Czech Republic;4. Research Unit Animal and Functionalities of Animal Products, USC INRA 340, University of Lorraine, 54505 Vandoeuvre-lès-Nancy, France;5. Slovak University of Agriculture in Nitra, Department of Animal Nutrition, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia |
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| Abstract: | The present study evaluated the effect of storage conditions on the LDL efficacy for cryopreserving ovine sperm. In this way, we compared egg yolk extender with three different forms for LDL storing, LDL diluted in Tris-glucose extender and stored in frozen (i) and freeze-dried (ii) states and LDL stored pure and added into the extender prior to use (iii). We also tested the effect of two storage temperatures (?20 and ?80 °C) and three storage times (30, 60, 120 d). Frozen and freeze-dried extenders containing LDL, as well as LDL stored pure, improved post-thaw sperm quality. Storage temperatures did not influence negatively the cryoprotectiveness of LDL extenders. Furthermore, lyophilised LDL extenders stored at ?20 °C were more effective in preserving sperm longevity than the other extenders stored at ?20 °C. Finally, LDL extenders stored for 30 and 120 d were more efficient than 60 d in preserving ram sperm freezability. |
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| Keywords: | Low-density lipoproteins Storage period Cryopreservation Lyophilisation Semen Ovine |
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