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The oxidant-antioxidant equilibrium,activities of selected lysosomal enzymes and activity of acute phase protein in peripheral blood of 18-year-old football players after aerobic cycle ergometer test combined with ice-water immersion or recovery at room temperature |
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| Institution: | 1. Department of Cardiology, Academic Medical Center — University of Amsterdam, Amsterdam, The Netherlands;2. Division of Molecular and Translational Cardiology, Department of Cardiology and Angiology, Hannover Medical School, Hannover, Germany;3. Department of Clinical Chemistry, Academic Medical Center — University of Amsterdam, Amsterdam, The Netherlands;1. Department of Cardiology and Pneumonology, Charité — Universitätsmedizin Berlin, Campus Benjamin Franklin, Berlin, Germany;2. IKDT (Institut für kardiale Diagnostik und Therapie), Moltkestrasse 31, Berlin, Germany;3. Department of Cardiology, Pneumonology and Intensive Care Medicine, Clinic for Internal Medicine I, University Hospital Jena, Friedrich-Schiller-University, Jena, Germany |
| Abstract: | The goal of the study was to evaluate the effect of an aerobic exercise bout followed by ice-water immersion or recovery at room temperature on the redox state, activities of selected lysosomal enzymes and activity of α1-antitrypsin (AAT) in the blood of healthy sportsmen. Eleven amateur football players aged 18 were randomly assigned to two similar 30-min aerobic cycle ergometer tests followed by a recovery at room temperature (20 °C; Experiment 1) or ice-water immersion (3 °C, 5 min; Experiment 2). Peripheral blood was collected three times during both study experiments: before (baseline), as well as 20 and 40 min after the recovery or immersion. The concentrations of thiobarbituric acid reactive substances in blood plasma (plTBARS) and erythrocytes (erTBARS) were measured. The erythrocytic activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were also determined. In the blood serum, the activities of acid phosphatase (AcP), arylsulphatase (ASA), cathepsin D (CTS D) and AAT were evaluated. The activities of AcP, ASA, CTS D and AAT changed similarly during both experiments. The GPx activity decreased 40 min after the exercise/recovery compared to the baseline activity and was lower than 40 min after the exercise/immersion. The exercise followed by the recovery or immersion had no significant effect on the serum lysosomal and AAT activities in the studied men. The exercise/recovery reduced the hydrogen peroxide concentration in the men's erythrocytes, however the exercise/immersion demonstrated the opposite effect. |
| Keywords: | Antioxidant enzymes Lysosomal enzymes Physical effort Reactive oxygen species Low temperature α1-Antitrypsin ROS"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"reactive oxygen species SOD"} {"#name":"keyword" "$":{"id":"kwrd0055"} "$$":[{"#name":"text" "_":"superoxide dismutase GPx"} {"#name":"keyword" "$":{"id":"kwrd0065"} "$$":[{"#name":"text" "_":"glutathione peroxidase CAT"} {"#name":"keyword" "$":{"id":"kwrd0075"} "$$":[{"#name":"text" "_":"catalase TBARS"} {"#name":"keyword" "$":{"id":"kwrd0085"} "$$":[{"#name":"text" "_":"thiobarbituric acid reactive substances MDA"} {"#name":"keyword" "$":{"id":"kwrd0095"} "$$":[{"#name":"text" "_":"malondialdehyde IWI"} {"#name":"keyword" "$":{"id":"kwrd0105"} "$$":[{"#name":"text" "_":"ice-water immersion DOMS"} {"#name":"keyword" "$":{"id":"kwrd0115"} "$$":[{"#name":"text" "_":"delayed-onset muscle soreness plTBARS"} {"#name":"keyword" "$":{"id":"kwrd0125"} "$$":[{"#name":"text" "_":"plasma TBARS erTBARS"} {"#name":"keyword" "$":{"id":"kwrd0135"} "$$":[{"#name":"text" "_":"erythrocytic TBARS AcP"} {"#name":"keyword" "$":{"id":"kwrd0145"} "$$":[{"#name":"text" "_":"acid phosphatase ASA"} {"#name":"keyword" "$":{"id":"kwrd0155"} "$$":[{"#name":"text" "_":"arylsulfatase CTS D"} {"#name":"keyword" "$":{"id":"kwrd0165"} "$$":[{"#name":"text" "_":"cathepsin D AAT"} {"#name":"keyword" "$":{"id":"kwrd0175"} "$$":[{"#name":"text" "_":"α1-antitrypsin BIA"} {"#name":"keyword" "$":{"id":"kwrd0185"} "$$":[{"#name":"text" "_":"bioelectrical impedance analysis IPAQ"} {"#name":"keyword" "$":{"id":"kwrd0195"} "$$":[{"#name":"text" "_":"international physical activity questionnaire maximum oxygen uptake PWC"} {"#name":"keyword" "$":{"id":"kwrd0215"} "$$":[{"#name":"text" "_":"physical working capacity RT"} {"#name":"keyword" "$":{"id":"kwrd0225"} "$$":[{"#name":"text" "_":"room temperature HRmax"} {"#name":"keyword" "$":{"id":"kwrd0235"} "$$":[{"#name":"text" "_":"maximal heart rate TBA"} {"#name":"keyword" "$":{"id":"kwrd0245"} "$$":[{"#name":"text" "_":"thiobarbituric acid GSH"} {"#name":"keyword" "$":{"id":"kwrd0255"} "$$":[{"#name":"text" "_":"reduced glutathione GSSG"} {"#name":"keyword" "$":{"id":"kwrd0265"} "$$":[{"#name":"text" "_":"oxidized glutathione NADPH"} {"#name":"keyword" "$":{"id":"kwrd0275"} "$$":[{"#name":"text" "_":"nicotinamide adenine dinucleotide phosphate 4-NCS"} {"#name":"keyword" "$":{"id":"kwrd0285"} "$$":[{"#name":"text" "$$":[{"#name":"__text__" "_":"4-"} {"#name":"italic" "_":"p"} {"#name":"__text__" "_":"-nitrocatechol 4-NC"} {"#name":"keyword" "$":{"id":"kwrd0295"} "$$":[{"#name":"text" "_":"4-nitrocatechol |
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