Effects of trehalose vitrification and artificial oocyte activation on the development competence of human immature oocytes |
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| Institution: | 1. Reproductive Medicine Center, Department of Obstetrics and Gynecology, First Affiliated Hospital of Anhui Medical University, Hefei 230022, China;2. Insititute of Reproduction and Genetics of Anhui Medical University, Hefei 230022, China;3. Biopreservation and Artifical Organs, Anhui Provincial Engineering Research Center, Hefei 230022, China;4. Center for Biomedical Engineering, Department of Electronic Science and Technology, University of Science and Technology of China, Hefei 230027, Anhui, China;1. Laboratory of Physiology and Control of Reproduction, State University of Ceará, Fortaleza, CE, Brazil;2. Faculty of Veterinary Medicine, Fluminense Federal University, Niterói, RJ, Brazil;3. INRA, Physiologie de la Reproduction et des Comportements, Nouzilly, France;1. Departamento de Ciencias de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 DF, Mexico;2. Doctorado en Ciencias Biológicas y de la Salud, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 DF, Mexico;3. Departamento de Biología de la Reproducción, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, 09340 DF, Mexico;4. Departamento de Medicina y Cirugía Animal, Universidad de Murcia, Espinardo 30100, Spain;1. Universidade Federal do Rio Grande do Sul (UFRGS), Programa de Pós-Graduação em Zootecnia, 91540-000, Porto Alegre, RS, Brazil;2. Universidade Estadual do Oeste do Paraná (UNIOESTE), Instituto de Pesquisa em Aqüicultura Ambiental (InPAA), 85900-030, Toledo, PR, Brazil;1. Department of Genetic , Shahid Sadoughi University of Medical Sciences, Yazd,Iran;2. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran;3. Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran;1. College of Veterinary Medicine, Nanjing Agricultural University, Jiangsu 210095, China;2. Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;3. Division of Animal Genetic Engineering, Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Shanghai 201106, China |
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| Abstract: | Sucrose and trehalose are conventional cryoprotectant additives for oocytes and embryos. Ethanol can artificially enhance activation of inseminated mature oocytes. This study aims to investigate whether artificial oocyte activation (AOA) with ethanol can promote the development competence of in vitro matured oocytes. A total of 810 human immature oocytes, obtained from 325 patients undergoing normal stimulated oocyte retrieval cycles, were in vitro maturated (IVM) either immediately after collection (Fresh group n = 291)) or after being vitrified as immature oocytes (Vitrified group n = 519). These groups were arbitrarily assigned. All fresh and vitrified oocytes which matured after a period of IVM then underwent intra-cytoplasmic sperm injection (ICSI). Half an hour following ICSI, they were either activated by 7% ethanol (AOA group) or left untreated (Non-AOA group). Fertilization, cleavage rate, blastocyst quality and aneuploidy rate were then evaluated. High-quality blastocysts were only obtained in both the fresh and vitrified groups which had undergone AOA after ICSI. Trehalose vitrification slightly, but not significantly, increased the formation rates of high-quality embryos (21.7% VS 15.4%, P > 0.05) and blastocysts (15.7% VS 7.69%, P > 0.05)) when compared with sucrose vitrification. Aneuploidy was observed in 12 of 24 (50%) of the AOA derived high quality blastocysts. High-quality blastocysts only developed from fresh or vitrified immature oocytes if the ICSI was followed by AOA. This information may be important for human immature oocytes commonly retrieved in normal stimulation cycles and may be particularly important for certain patient groups, such as cancer patients. AOA with an appropriate concentration of ethanol can enhance the developmental competence of embryos. |
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| Keywords: | Trehalose vitrification Artificial oocyte activation (AOA) Human immature oocytes Aneuploidy Fluorescence in situ hybridization (FISH) |
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