Long-term preservation of eri and ailanthus silkworms using frozen gonads |
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| Institution: | 1. Silkworm Resource Division, Institute of Genetic Resources, Kyushu University Graduate School of Bio Resources and Bioenvironmental Science, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan;2. Graduate School of Agricultural and Life Science, University of Tokyo, Bunkyo, Tokyo 113-8657, Japan;3. Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan;1. Department of Dental Research, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming, Yunnan 650500, PR China;2. National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu 610041, PR China;3. Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, College of Stomatology, Chongqing Medical University, Chongqing, 401147, PR China;4. Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, PR China;5. Department of Orthodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming, Yunnan 650031, PR China;1. Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, USA;2. Department of Engineering, East Carolina University, Greenville, NC, USA;3. Institute for Engineering in Medicine, University of Minnesota, Minneapolis, MN, USA;4. Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USA;1. Department of Heart Failure and Cardiac Rehabilitation, Second Faculty of Medicine, Medical University of Warsaw, Poland;2. Department of Experimental and Clinical Physiology, Warsaw Medical University, Poland;3. Chair and Department of Cardiology, Hypertension and Internal Diseases, Second Faculty of Medicine, Medical University of Warsaw, Poland;1. Clinica Santa Elena, Malaga, Spain;2. Inter Science GmbH, Biophysics, Luzern, Switzerland;3. Instituto Andaluz Cirugía Experimental IACE, Malaga, Spain;4. Hippocrates DOO, Divaca, Slovenia;5. Pathology Research Laboratory, Inc, 521 Rocca Avenue, South San Francisco, CA 94080, USA;6. Department of Mechanical Engineering, University of California, Berkley, CA 94720, USA |
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| Abstract: | Cryopreservation of eri and ailanthus silkworms using frozen gonads was investigated. First, we evaluated the freeze tolerance of ovary and testis in the eri silkworm, which showed high tolerance. Mating between frozen ovary-transplanted females and frozen testis-transplanted males produced 163.0 eggs, yielding 105.7 larvae per moth. In a second experiment, we tested the use of the eri silkworm as a host insect for gonad transplantation from ailanthus silkworm donors. A high success ratio for laid and hatched eggs was demonstrated for ovary transplantation (97.8 and 51.3 eggs per moth, respectively). For testis transplantation, however, the average number of hatched larvae was low (12.0). Mating between host eri females and males in which both frozen ovary and testis of the ailanthus silkworm had been transplanted produced 6.4 fertilized eggs per host moth. Our success in using cross subspecies cryopreservation between these wild silkworms could lead to the alternative use of hosts between species in other insects. |
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| Keywords: | Cryopreservation Ovary Testis Transplantation Eri silkworm Ailanthus silkworm Cross-species cryopreservation |
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