Enzyme Inhibition and Antioxidant Activities of Asparagus officinalis L. and Analysis of Its Phytochemical Content by LC/MS/MS |
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| Authors: | Prof. Dr. Veysel Çomaklı Dr. Rüya Sağlamtaş Prof. Dr. Müslüm Kuzu Dr. Yalçın Karagöz Assoc. Prof. Dr. Tuba Aydın Prof. Dr. Ramazan Demirdağ |
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| Affiliation: | 1. Department of Nutrition and Dietetics, İbrahim Çeçen University of Ağrı, Ağrı, Türkiye;2. Department of Medical Services and Techniques, İbrahim Çeçen University of Ağrı, Ağrı, Türkiye;3. Department of Nutrition and Dietetics, Karabük University, Karabük, Türkiye;4. Department of Pharmaceutical Botany, İbrahim Çeçen University of Ağrı, Ağrı, Türkiye;5. Department of Pharmacognosy, İbrahim Çeçen University of Ağrı, Ağrı, Türkiye |
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| Abstract: | In the study, water, ethanol, methanol, dichloromethane, and acetone extracts of Asparagus officinalis L. were obtained by maceration. DPPH⋅, ABTS⋅+, FRAP, and CUPRAC methods determined the antioxidant capacities of all extracts. Moreover, the in vitro effects of extracts on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase (CA)-I, CA-II and α-Glycosidase were investigated. At a 10 μg/ml concentration, the extract with the highest Fe3+ reduction capacity was ethanol (AE), and the extract with the highest Cu2+ reduction capacity was acetone (AA). AE for AChE (IC50=21.19 μg/ml) and α-Glycosidase (IC50: 70.00 μg/ml), methanol (AM) for BChE (IC50=17.33 μg/ml), CA−I and II (IC50=79.65 and 36.09 μg/ml, respectively) showed the most potent inhibition effect. The content analysis of acetone extract was performed with LC/MS-MS, the first three phytochemicals found most were p-Coumaric acid, rutin, and 4-hydroxybenzoic acid (284.29±3.97, 135.39±8.19, and 102.06±5.51 μg analyte/g extract, respectively). |
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| Keywords: | asparagus officinalis cholinesterase carbonic anhydrase α-glycosidase inhibition |
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