Optimization of the first dimension for separation by two-dimensional gel electrophoresis of basic proteins from human brain tissue |
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| Authors: | Pennington Kyla McGregor Emma Beasley Clare L Everall Ian Cotter David Dunn Michael J |
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| Institution: | Department of Psychological Medicine, Institute of Psychiatry, UK. k.pennington@iop.kcl.ac.uk |
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| Abstract: | A major cause of poor resolution in the alkaline pH range of two-dimensional electrophoresis (2-DE) gels is unsatisfactory separation of basic proteins in the first dimension. We have compared methods for the separation of basic proteins in the isoelectric focusing dimension of human brain proteins. The combined use of anodic cup-loading and the hydroxyethyldisulphide containing solution (DeStreak) produced better resolution in both analytical and micropreparative protein loaded 2-DE gels than the other methods investigated. |
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| Keywords: | Basic proteins DeStreak Human brain Isoelectric focusing Two-dimensional gel electrophoresis |
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