DNA sequence analysis of X-ray-induced deletions at the white locus of Drosophila melanogaster |
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Authors: | A Pastink C Vreeken A P Schalet J C Eeken |
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Institution: | Department of Radiation Genetics and Chemical Mutagenesis, State University of Leiden, The Netherlands. |
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Abstract: | We have determined the nucleotide sequence of 5 X-ray-induced white mutants containing small rearrangements. Comparison with wild-type sequences showed deletions in the coding region ranging in size between 6 bp and 29 bp. These small deletions are distributed non-randomly over the white locus. Two mutants contain the same 29-bp deletion, while the other 3 deletions are clustered. All 5 deletions have occurred between 2 and 3 bp repeats. One of the repeats is preserved in the novel junction formed by the deletion. Our results suggest that recombinational processes may be involved in the generation of X-ray-induced deletions. |
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