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Mapping of Rice Rf Gene by Bulked Line Analysis |
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| Authors: | Tan, Xue-Lin Vanavichit, Apichart Amornsilpa, Sommai Trangoonrung, Somvong |
| Affiliation: | 1Tropical Agriculture, Faculty of Agriculture, Kasetsart University Thailand 2Agricultural Genetic Engineering and Biotechnology Center, Research and Development Institute, Kasetsart University Nakhon Pathom 73140, Thailand 3Genetic Division, Rice Research Institute, Department of Agriculture Bangkok 10900, Thailand 4DNA fingerprinting Unit, National Center for Genetic Engineering Unit Nakhon Pathom 73140, Thailand |
| Abstract: | A method, bulked line analysis (BLA), was developed for identificationof the RFLP markers associated with a target gene. Instead ofsegregating progenies, conventional lines sharing the same traitwere bulked by the BLA method. This method is an alternativeapproach to the identification of DNA markers linked with atarget gene. A major advantage of this method is time-savingfor genetic stock development. The advantage is very significantfor organisms having a long generation period. This method hasbeen tested by using fertility restoration of rice cytoplasmicmale sterility of wild abortive type as a target trait. A fertility-restoringgene was successfully identified by linkage with RFLP markers.This gene was mapped in the middle of the long arm of chromosome10 of the rice genome. |
| Keywords: | Rf gene Gene Mapping Marker Identification RFLP marker |
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