番茄褪绿病毒对Q型烟粉虱重要生物学参数及保护酶和解毒酶活力的影响

为明确番茄褪绿病毒(ToCV)对其传毒介体烟粉虱Bemisia tabaci主要生物学特性的影响,本文研究了携带ToCV的Q型烟粉虱在非病毒寄主植物棉花Gossypium spp上的生物学指标,并测定了带毒和无毒烟粉虱主要保护酶和解毒酶活性.结果 表明,在棉花植株上,带毒烟粉虱在发育历期、产卵量、成虫寿命方面与无毒烟粉虱无显著差异,但雌虫体长明显短于无毒烟粉虱.相对于无毒烟粉虱,带毒烟粉虱体内过氧化氢酶(catalase,CAT)活性明显提高,是无毒烟粉虱的3.36倍(P ＜0.001),超氧化物歧化酶(superoxide dismutase,SOD)和过氧化物酶(peroxidase,POD)活性无显著差异.解毒酶中,带毒烟粉虱羧酸酯酶(carboxylesterase,CarE)活性明显下降,是无毒烟粉虱活性的54％,谷胱甘肽S转移酶(glutathione-s-transferase,GST)和乙酰胆碱酯酶(acetylcholin esterase,ACHE)活性无显著差异.

Impact of ToCV on important biological parameters, and activities of protective and detoxification enzymes in Bemisia tabaci Q

To explicit the impact of Tomato chlorosis virus (ToCV) on main biological traits of its vector Bemisia tabaci, the biological parameters of B.tabaci Q infected by ToCV were detected on cotton (a non-host of the virus), and activities of main protective and detoxification enzymes were detected in B.tabaci Q virus infected and non-infected one. The results showed that the developmental period, fecundity and longevity were not different apparently between infected and non-infected B.tabaci. The body size of female adult of infected ones was shorter than non-infected ones. Compared to non-infected B.tabaci, activities of catalase (CAT) in infected B.tabaci increased to 3.36 times (P<0.001), while activity of superoxide dismutase (SOD) and peroxidase (POD) in infected B.tabaci didn''t change significantly. In detoxification enzymes, carboxylesterase (CarE) activity in infected B-tabaci decreased significantly and which was only 54% of that of non-infected ones. Glutathione-s-transferase (GST) and acetylcholin esterase (AChE) showed no difference between infected and non-infected B.tabaci.