Repair effect of diabetic ulcers with recombinant human epidermal growth factor loaded by sustained-release microspheres |
| |
Authors: | XiaoQing Dong Jun Xu WeiCai Wang Hao Luo XiaoFei Liang Lei Zhang HanJie Wang PengHua Wang Jin Chang |
| |
Institution: | 1.Institute of Nanobiotechnology, School of Materials Science and Engineering,Tianjin University,Tianjin,China;2.Department of Diabetic Foot,Tianjin Medical University Metabolic Disease Hospital,Tianjin,China |
| |
Abstract: | In this study the w/o/w extraction-evaporation technique was adopted to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres loading recombinant human epidermal growth factor (rhEGF). The microspheres were characterized
for morphology by transmission electron microscopy (TEM) and particle size distribution. The release performances, the proliferation
effects and therapeutic effects of rhEGF-loaded PLGA microspheres were all studied. The results showed that these spherical
microspheres had a narrow size distribution and a high drug encapsulation efficiency (85.6%). RhEGF-loaded microspheres enhanced
the growth rate of fibroblasts and wound healing more efficiently than pure rhEGF. The number of the proliferating cell nuclear
antigen (PCNA) in the epidermis layer with the microsphere treatment was significantly larger than those of the control groups.
Overall locally sustained delivery of rhEGF from biodegradable PLGA microspheres may serve as a novel therapeutic strategy
for diabetic ulcer repair. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|