Endogenous abscisic acid and protein contents during seed development of <Emphasis Type="Italic">Araucaria angustifolia</Emphasis> |
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| Authors: | V Silveira C Santa-Catarina T S Balbuena F M S Moraes C A O Ricart M V Sousa M P Guerra W Handro E I S Floh |
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| Institution: | 1.Biotechnology Laboratory,CBB-UENF,Campos dos Goytacazes-Rio de Janeiro,Brazil;2.Department of Botany,IB-University of S?o Paulo, CP 11461,S?o Paulo-SP,Brazil;3.Department of Cell Biology,IB-University of Brasilia,Brasília-DF,Brazil;4.Department of Plant Science,CCA-Federal University of Santa Catarina,Florianópolis-SC,Brazil |
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| Abstract: | This paper describes a proteome analysis and changes in endogenous abscisic acid (ABA) contents during seed development of
Araucaria angustifolia (Bert.) O. Ktze. Megagametophytes and embryonic axis tissues exhibited a similar ABA variation pattern during seed development,
reaching maximum values at the pre-cotyledonary stage. The embryonic axis protein content increased until the cotyledonary
stage with following stabilization at mature seed. The two-dimensional electrophoresis at the torpedo developmental stage
showed approximately 230 polypeptides against 340 in the mature stage. Peptide mass fingerprinting analyses identified three
polypeptides, corresponding to an AtSAC4, a late embryogenesis abundant (LEA) and a storage protein, respectively. |
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