Cellulosic alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans endoglucanase and Saccharomycopsis fibuligeraβ-glucosidase |
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Authors: | Eugene Jeon Jeong eun Hyeon Lee Sung Eun Byeoung-Soo Park Seung Woo Kim Jinwon Lee & Sung Ok Han |
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Institution: | College of Life Science and Biotechnology, Korea University, Seoul, Korea;;Research Station, Nanotoxtech Inc., Ansan, Gyeonggi-do, Korea;;Department of Chemical and Biomolecular Engineering, Korea University, Seoul, Korea;;and Department of Chemical and Biomolecular Engineering, Sogang University, Seoul, Korea |
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Abstract: | In this study, Saccharomyces cerevisiae was engineered for simultaneous saccharification and fermentation of cellulose by the overexpression of the endoglucanase D (EngD) from Clostridium cellulovorans and the β-glucosidase (Bgl1) from Saccharomycopsis fibuligera . To promote secretion of the two enzymes, the genes were fused to the secretion signal of the S. cerevisiae α mating factor gene. The recombinant developed yeast could produce ethanol through simultaneous production of sufficient extracellular endoglucanase and β-glucosidase. When direct ethanol fermentation from 20 g L?1β-glucan as a substrate was performed with our recombinant strains, the ethanol concentration reached 9.15 g L?1 after 50 h of fermentation. The conversion ratio of ethanol from β-glucan was 80.3% of the theoretical ethanol concentration produced from 20 g L?1β-glucan. In conclusion, we have demonstrated the construction of a yeast strain capable of conversion of a cellulosic substrate to ethanol, representing significant progress towards the realization of processing of cellulosic biomass in a consolidated bioprocessing configuration. |
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Keywords: | Clostridium cellulovorans Saccharomyces cerevisiae noncellulosomal endoglucanase β-glucosidase cellulose degradation ethanol production |
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