Classification of cultivated rices into indica and japonica types by the isozyme,RFLP and two milled-rice methods |
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Authors: | A. P. Resurreccion C. P. Villareal A. Parco G. Second B. O. Juliano |
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Affiliation: | (1) Plant Breeding, Genetics, and Biochemistry Division, The International Rice Research Institute, P. O. Box 933, 1099 Manila, Philippines;(2) Present address: Centro National de Recursos Geneticos e Biotechnologia, Sain, CEP 70770 Brasilia DF, Brasil;(3) Present address: Institute of Chemistry, and Philippine Rice Research Institute, Division of Rice Chemistry and Food Science, University of the Philippines at Los Baños, 4031 College, Laguna, Philippines |
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Abstract: | Four methods for classifying cultivated rices (Oryza sativa L.) (including IR varieties) into indica and japonica types — waxy gene product in endosperm starch, glutelin 3 molecular weight in milled rice, RFLP polymorphism at the Wx locus and Glaszmann's isozyme method — were compared. On the basis of the two endosperm traits and the RFLP method Glaszmann's group 1 (indica) was classified as mainly indica and intermediate groups 2, 3 and 4 as exclusively indica. However, the endosperm traits classified Glaszmann's group 5 as mainly indica, while the RFLP method classified it as japonica. The RFLP waxy gene probe was closest to the isozyme method in classifying group 6 as japonicas; the waxy gene product gave mainly indica reaction even in group 6, and the glutelin 3 method was intermediate. All IR rices were classified as being indica on the basis of Wx gene product and by Glaszmann's method, but a few were classified as japonica by the glutelin 3 method and by the RFLP waxy gene probe. |
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Keywords: | Oryza sativa L. Waxy gene product Glutelin /content/n694160u7r220520/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >3 molecular weight Isozyme type RFLP waxy gene probe |
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