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Sources of nonlinearity in cDNA microarray expression measurements |
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| Authors: | Latha Ramdas Kevin R Coombes Keith Baggerly Lynne Abruzzo W Edward Highsmith Tammy Krogmann Stanley R Hamilton Wei Zhang |
| Institution: | (1) Department of Pathology, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA;(2) Department of Biostatistics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA;(3) Department of Biomathematics, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA;(4) Department of Hematopathology, Cancer Genomics Core Laboratory, University of Texas M D Anderson Cancer Center, Houston, TX 77030, USA;(5) Department of Pathology, University of Maryland, College Park, MD 20742, USA; |
| Abstract: | BackgroundA key assumption in the analysis of microarray data is that the quantified signal intensities are linearly related to the expression levels of the corresponding genes. To test this assumption, we experimentally examined the relationship between signal and expression for the two types of microarrays we most commonly encounter: radioactively labeled cDNAs on nylon membranes and fluorescently labeled cDNAs on glass slides. |
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