How Proteins Recognize the TATA Box |
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| Institution: | 1. Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA;2. Department of Chemistry, University of California at Los Angeles, Los Angeles, CA 90095, USA;3. Department of Microbiology and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095, USA |
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| Abstract: | The crystal structure of a complex of human TATA-binding protein with TATA-sequence DNA has been solved, complementing earlier TBP/DNA analyses fromSaccharomyces cerevisiaeandArabidopsis thaliana. Special insight into TATA box specificity is provided by considering the TBP/DNA complex, not as a protein molecule with bound DNA, but as a DNA duplex with a particularly large minor groove ligand. This point of view provides explanations for: (1) why T·A base-pairs are required rather than C·G; (2) why an alternation of T and A bases is needed; (3) how TBP recognizes the upstream and downstream ends of the TATA box in order to bind properly; and (4) why the second half of the TATA box can be more variable than the first. |
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