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| 犬2型腺病毒E3区的克隆及其缺失改造 | |
| 引用本文： | 邱薇,夏咸柱,范泉水,扈荣良,何洪彬,余春,王雷,高玉伟,张守峰.犬2型腺病毒E3区的克隆及其缺失改造[J].生物技术,2002,12(5):6-8. |
| 作者姓名： | 邱薇 夏咸柱 范泉水 扈荣良 何洪彬 余春 王雷 高玉伟 张守峰 |
| 作者单位： | 1. 成都军区联勤部军事医学研究所,云南,昆明,650032 2. 解放军军需大学军事兽医研究所,吉林,长春,130062 3. 东北农业大学,黑龙江,哈尔滨,150030 4. 北京军区军犬队,北京,100021 |
| 基金项目： | 全军“十五”医药卫生重点项目基金资助项目 (0 1 -Z - 0 92 )，国家自然科学基金项目 (30 1 70 70 4 ) |
| 摘 要： | |
| 关 键 词： | 犬2型腺病毒 E3区 克隆 缺失改造 |
| 文章编号： | 1004-311X(2002)05-0006-03 |
| 修稿时间： | 2002年6月10日 |
Clone and deletion of the E3 region of SY strain of CVA-2 |
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| QIU Wei,XIA Xian-zhu,FAN Quan-shui,HU Rong-liang,HE Hong-bin,YU Chun,WANG Lei,GAO Yu-wei,ZHANG Shou-feng.Clone and deletion of the E3 region of SY strain of CVA-2[J].Biotechnology,2002,12(5):6-8. | |
| Authors: | QIU Wei XIA Xian-zhu FAN Quan-shui HU Rong-liang HE Hong-bin YU Chun WANG Lei GAO Yu-wei ZHANG Shou-feng |
| Institution: | QIU Wei1,XIA Xian-zhu2,FAN Quan-shui1,HU Rong-liang2,HE Hong-bin3,YU Chun4,WANG Lei2,GAO Yu-wei2,ZHANG Shou-feng2 |
| Abstract: | To construct the CAV-2 E3 region expressing vector for foreign genes, the E3 region of the SY strain of CAV-2 was cloned by enzyme digestion and then sequensed. Two pairs of mutation primers were designed according to the sequences of the two ends of the E3 region. After two times of mutation, two Bgl II sites were introduced into the recombinent plasmid. A 1.4kb fragment of E3 region was deleted through the digestion of Bgl II and a linker with 5 single enzyme sites was inserted into the Bgl II site. The plasmid can be used to construct E3 expressing vectors of CAV-2. |
| Keywords: | canine adenovirus 2 E3 region clone deletion |
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