Photopolymerization as an innovative detection technique for low-density microarrays |
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Authors: | Kuck Laura R Taylor Amber W |
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Institution: | InDevR, Inc., 2100 Central Ave., Suite 106, Boulder CO 80301, USA. lkuck@indevr.net |
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Abstract: | One limitation that accounts in part for the scarcity of commercially available diagnostic microarrays is the expense associated with fluorescence detection. Here we present a colorimetric method based on photopolymerization as an "on-chip" signal amplification technique. Proof of principle experiments are detailed and followed by the use of a simple influenza microarray to demonstrate the technique for the first time with clinical samples. The advantages of this new technique include rapid (<5 min) signal amplification ( approximately 105) in ambient conditions for both DNA and protein microarrays, low reagent cost (<$1 per assay), visual or inexpensive detection, and signal preservation for at least two years under ambient conditions. |
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