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General transduction in Rhizobium meliloti by a thermosensitive mutant of bacteriophage DF2. |
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| Authors: | J Casadesú s J Olivares |
| Affiliation: | J Casadesús and J Olivares |
| Abstract: | A simiplified version of bacteriophage DF2 transduction, using the thermosensitive mutant DF2tsC, is described. The transduction frequencies of Rhizobium meliloti markers by DF1tsC are of about 10(-6) or higher. The development of transductants at 34 degrees C does not require the addition of antiserum for phage neutralization. |
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