Transformations in isolated polyhooks. |
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Authors: | H Kagawa S I Aizawa S Asakura |
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Affiliation: | Institute of Molecular Biology Faculty of Science Nagoya University, Nagoya, 464 Japan |
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Abstract: | Polyhooks from certain non-flagellated mutants of Salmonella and Escheriohia coli are known to be helix-shaped under physiological conditions. However, two non-helical forms were found to occur in isolated polyhooks under appropriate conditions. One form is straight, and appeared when the pH was decreased to 3.2 or lower. The helical-to-straight transformation was reversible. The other form is a doughnut with a fairly constant diameter of above 0.1 μm, which was produced when polyhooks were heated at 75 °C in the absence of salts. |
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