A method for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum |
| |
| Authors: | Barbara C. Dill John J. Stock |
| |
| Affiliation: | 1. Department of Microbiology, The University of British Columbia, Vancouver, British Columbia
|
| |
| Abstract: | A method is reported for the isolation of nuclei from the dermatophytic fungus,Microsporum gypseum. The yield ranged from greater than 90% in crude preparations to less than 20% in highly purified preparations as judged by the recovery of DNA. Nuclei isolated in this manner appeared intact in electron microscopic observations and possessed chemical compositions, i.e. low protein/DNA and RNA/DNA ratios, which indicated a low degree of cytoplasmic contamination. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
