A human transferrin-binding protein of Staphylococcus aureus is immunogenic in vivo and has an epitope in common with human transferrin receptor |
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| Authors: | Y. Lim S.H. Shin I.Y. Jang J.H. Rhee I.S. Kim |
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| Affiliation: | Department of Microbiology, Chosun University Medical School, Seosuk-Dong, Dong-Gu, Kwangju 501-759, South Korea;Department of Anatomy, Chosun University Medical School, Seosuk-Dong, Dong-Gu, Kwangju 501-759, South Korea;Department of Microbiology, Chonnam National University, Medical School, Kwangju 501-190, South Korea;Department of Microbiology, Seoul National University Medical school, Seoul 110-460, South Korea |
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| Abstract: | To understand human immune responses against the human transferrin-binding protein of Staphylococcus aureus (SA-tbp), we examined cell wall proteins from S. aureus ATCC 6538 using human convalescent sera, and a monoclonal antibody specific for human transferrin receptor (McAb-HTR). The SA-tbp, detected by immunoblot assay, was iron-repressible, reacted with the convalescent sera, and cross-reacted with McAb-HTR. Immunoelectron microscopy probed with McAb-HTR showed a reaction zone around the test strain from the deferrated BHI. After being preincubated with an S. aureus -bacteremic serum, the electroblot of the SA-tbp still reacted with McAb-HTR, but not with human transferrin-horseradish peroxidase conjugate. We conclude, there are at least two kinds of epitopes in the SA-tbp; one able to bind to human transferrin is immunogenic in humans, but the other sharing epitopes common with human transferrin receptor is not immunogenic in humans. |
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| Keywords: | Staphylococcus aureus Iron Human transferrin Transferrin-binding protein Transferrin receptor |
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