Study of different coupling agents in the conjugation of a V3-based synthetic MAP to carrier proteins. |
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Authors: | L J Cruz E Iglesias J C Aguilar D Quintana H E Garay C Duarte O Reyes |
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Institution: | División de Química-Física, Centro de Ingeniería Genética y Biotecnologia, Cubanacán, Habana, Cuba. ljcruz@cigb.edu.cu |
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Abstract: | The conjugation of synthetic peptides to carrier proteins is a widely used method for immunological studies. Different coupling agents have been described to form the conjugate with carrier proteins. In this paper, we demonstrate that the antibody response toward V3-based synthetic MAPs derived from HIV-1, JY1 isolate, conjugated to two different carrier proteins using either m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) or beta-maleimidopropionic acid N-hydroxysuccinimide ester (MPS), or succinic anhydride (SA) show different behaviors. An excellent anti-JY1 response without a strong response to the coupling agent is observed in the case of succinic anhydride spacer. In contrast, MBS produces total abrogation of the antibody response with a high response toward the coupling agent. |
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Keywords: | anti-peptide antibody m-maleimidobenzoyl-N-hydroxysuccinimide ester β-maleimidopropionic acid N-hydroxysuccinimide ester peptide conjugate succinic anhydride |
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