Biomass and lipid production of heterotrophic microalgae <Emphasis Type="Italic">Chlorella protothecoides</Emphasis> by using biodiesel-derived crude glycerol |
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Authors: | Yen-Hui Chen Terry H Walker |
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Institution: | (1) Biosystems Engineering, Clemson University, Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634, USA |
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Abstract: | Microalgal lipids may be a more sustainable biodiesel feedstock than crop oils. We have investigated the potential for using
the crude glycerol as a carbon substrate. In batch mode, the biomass and lipid concentration of Chlorella protothecoides cultivated in a crude glycerol medium were, respectively, 23.5 and 14.6 g/l in a 6-day cultivation. In the fed-batch mode,
the biomass and lipid concentration improved to 45.2 and 24.6 g/l after 8.2 days of cultivation, respectively. The maximum
lipid productivity of 3 g/l day in the fed-batch mode was higher than that produced by batch cultivation. This work demonstrates
the feasibility of crude biodiesel glycerol as an alternative carbon substrate to glucose for microalgal cultivation and a
cost reduction of carbon substrate feed in microalgal lipid production may be expected. |
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