In vitro cultures of Drosera aliciae as a source of a cytotoxic naphthoquinone: ramentaceone |
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| Authors: | Kawiak Anna Królicka Aleksandra Łojkowska Ewa |
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| Affiliation: | (1) Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdansk & Medical University of Gdansk, Kładki 24, 80-822 Gdansk, Poland;(2) Laboratory of Human Physiology, Faculty of Health Sciences with Subfaculty of Nursing, Medical University of Gdansk, Tuwima 15, 80-210 Gdansk, Poland |
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| Abstract: | A protocol for the in vitro propagation of Drosera aliciae to increase the yield of the naphthoquinone, ramentaceone, was developed. The highest micropropagation coefficient was obtained using half-strength Murashige–Skoog medium supplemented with 0.4 μM 6-benzyladenine (BA). The genetic fidelity and stability of the regenerated plants was confirmed with RAPD markers. The activity of the isolated ramentaceone was determined against four human tumor cell lines: U937, HeLa, MCF-7, HCT-116 with the highest cytotoxic activity towards the leukemic U937 cell line with an IC50 value of 3.2 μM. |
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