An interspecific somatic hybrid between upland cotton (G. hirsutum L. cv. ZDM-3) and wild diploid cotton (G. klotzschianum A.) |
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Authors: | Yuqiang Sun Shoumei Liu Yu Wang Brian Joseph Jones Huizhong Wang Shuijin Zhu |
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Affiliation: | (1) College of Life and Environmental Science, Hangzhou Normal University, Zhejiang, China;(2) College of Agriculture and Biotechnology, Zhejiang University, 310029 Hangzhou, People’s Republic of China;(3) Faculty of Agriculture, Food and Natural Resources, The University of Sydney, A20 John Woolley Building, Sydney, NSW, 2006, Australia |
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Abstract: | Somatic hybrids were produced through protoplast electrofusion between the tetraploid cotton Gossypium hirsutum L. cv. ZDM-3 and the wild diploid cotton G. klotzschianum. Hybrid plants were generated from 3 out of 24 callus lines that were derived from fused protoplasts. Hybrid plants were initially identified as somatic hybrids by ploidy analysis: the plants from the 3 callus lines had chromosome numbers near to sum of the two parents (78 = 52 + 26). The plants from the 3 lines were subsequently confirmed as hybrids by cytological, molecular, histological and morphological analyses. The morphology of hybrids was distinct from that of the parents, with elongated stigmas and malformed anthers lacking microspores and pollen, leading to male sterility. It is expected that the male sterility resulted from the high number of univalent and irregular multivalent chromosome pairings per meiocyte. |
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