Changes in the pattern of phospholipid synthesis during the induction by cytokinin of cell division in soybean suspension cultures |
| |
Authors: | R J A Connett D E Hanke |
| |
Institution: | (1) Botany School, Downing Street, CB2 3EA Cambridge, UK;(2) Present address: Unit 154/155, Agricultural Genetics Company, Cambridge Science Park, Cambridge, UK |
| |
Abstract: | A method is described for preparing fully viable, cytokinin-starved soybean (Glycine max (L.) Merr. cv. Acme) cells from a suspension-culture of callus tissue. The cells respond to kinetin treatment by re-initiating cell division. We present evidence, from the pattern of incorporation of 32P-labelled inorganic phosphate into individual phospholipids during the first hour of this response, that the synthesis of phosphatidylinositol (PI) and of phosphatidic-acid head-groups is affected within 15 min. The polyphosphoinositide phosphatidylinositol 4-phosphate, but not phosphatidylinositol 4,5-bisphosphate, was detected in the tissue. The characteristics of cytokinin-induced PI synthesis in cytokinin-starved soybean cells appear to resemble the PI response of animal cells.Abbreviations DPG
diphosphatidylglycerol
- PA
phosphatidic acid
- PC
phosphatidylcholine
- PE
phosphatidylethanolamine
- PG
phosphatidylglycerol
- PI
phosphatidylinositol
- PIP
phosphatidylinositol 4-phosphate
- PIP2
phosphatidylinositol 4,5-bisphosphate
- PS
phosphatidylserine
- Pi
inorganic phosphate
- TLC
thin-layer chromatography |
| |
Keywords: | Cell culture (cytokinin and cell division) Cell division Cytokinin and cell division Glycine (cell culture) Phosphatidylinositol |
本文献已被 SpringerLink 等数据库收录! |
|