cDNA Cloning and Structural Analysis of Rice ADP-Glucose Pyrophosphorylase Gene

ADP-glucose pyrophosphorylase is a key enzyme in the pathway of starch synthesis. The gene encoding this.important enzyme was cloned by PCR amplification from immature seeds of a Chinese rice cultivar-Oryza sativa var. japonica cv. Zhonghua 10. It was then sequenced and compared with the same gene reported from other rice cultivar. The gene obtained in this study is composed of 1461 bp and encodes 483 amino acid residues. Its nucleotide sequence and deduced amino acid sequence shared 99.6% and 99.7% homology with those of published data respectively. The analyses on the structure and evolution of this gene have been conducted.