Isolation and Characterization of Phosphatase from Thylakoid Membranes of Spinach Chloroplasts |
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Authors: | Wang Guo-qiang Peng Jian-xin Qian Yue-qin Li Rong Zhang Zheng-fu |
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Abstract: | A phosphatase from thylakoid membrane of spinach (Spinacia oleracea L. ) chloroplasts was isolated with the methods of extraction with n-ButanoL centrifugation at 100000 g for 30 min and chromatographic separation through DEAE-Cellulose (DE 52) column.The phosphatase catalyzed hydrolysis of phosphate monoesters (4-nitrophenyl phosphate). The optimal pH for enzyme catalysis was below 7. The peak rate of the enzyme reaction was obtained when it was incubated at 60℃ for 15 min. The phosphatase was inhibited by ATP and phosphate. The results from SDS-PAGE showed that the preparation of enzyme wascomposed of two proteins. |
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Keywords: | Phosphatase 4-nitrophenyl phosphate Thylakoid membrane ATP |
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