Genetic Transformation of Brassica juncea var. tsatsi Induced by Ri T-DNA of Agrobacterium rhizogenes |
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Authors: | Huang Ju-hui Zhou Chang-jiu Qin Feng-qin Wang Fu-jun |
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Abstract: | Hairy roots of mustard (Brassica juncea var. tsatsi) cv. "Paoye' were obtained from in vitro inoculation of reversely inserted petioles with Agrobacteriurn rhizogenes strain LBA9402 harbouring the agropine-type Ri plasmid (pRi1855). The root inducing rate was 100%. Transformed roots grew rapidly on hormone-free MS medium and showed typical hairy root phenotype. Transformed plantlets regenerated from hairy roots on MS medium supplemented with BA 8.0 mg/L and NAA 0.6 mg/L. Opine analysis evidenced the integration and expression of TR-DNA, PCR analysis and Southern hybridization confirmed the integration of TL-DNA including 862 bp rol B sequence in the transformed plants. |
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Keywords: | Agrobacterium rhizogenes Brassica juncea var. tsatsi Hairy root Poly.merase chain reaction Transgenic plant |
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