Cloning and Sequencing of Coat Protein Gene of Papaya Ringspot Virus and Sequence Comparison of Strains of PRV-P and PRV-YS

Papaya rinspot virus (PRV) was isolated and purified from infected papaya (Carica papaya L. )leaves in Hainan Island. The first strand of cDNA was synthesized with Olig(dT)as a primer. The coat protein gene of PRV was obtained by PCR techniques with primers synthesized accoding to the DNA sequence of PRV-P. Full length of cDNA clone encoding coat protein of PRV was sequenced and analysed. The result shows that the strain of PRV we isolated contains 881 nucleotides with 287 amino acids. Sequences among several strains of PRV were compared and it indicates an over 90% homology in DNA sequence of PRV-G the strain we isolated with PRV-P and PRV-YS. Highly convered sequence was located in carboxyl end and interestingly highly variable region was in N-terminal.