Construction of a Rubisco Fusion Gene and Its Expression in E. coli

A fusion gene was constructed with the Signal sequence of Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) small subunit from tomato and the coding region of Rubisco large subunit from spinach. The fusion gene was confirmed with restriction endonucleases and DNA sequencing analysis for the open reading frame. The chimeric gene was transferred to E. coli and its expression was induced by addition of IPTG. Expression of the Rubisco fusion gene was detected by Western blotting.